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Home / ELISA Kit

Mouse Syntaxin-1A (STX1A) ELISA Kit

SKU : BHE13705436

Syntaxin-1A is a protein encoded by the STX1A gene.Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone.
Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor which produces explosive exocytosis .Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Mfr.No.	KTE70276
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Syntaxin-1A (STX1A) ELISA Kit employs a two-site sandwich ELISA to quantitate STX1A.
Alternative Names	STX1A; HPC-1; STX1; p35-1; syntaxin 1A
Detection Method	Colorimetric
Kit Components	Mouse Syntaxin-1A microplate Mouse Syntaxin-1A standard Mouse Syntaxin-1A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Syntaxin-1A (STX1A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse STX1A. No significant cross-reactivity or interference between Mouse STX1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Syntaxin-1A is a protein encoded by the STX1A gene.Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor which produces explosive exocytosis .Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Notes	This Mouse Syntaxin-1A (STX1A) ELISA Kit employs a two-site sandwich ELISA to quantitate STX1A in samples. An antibody specific for STX1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTX1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STX1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STX1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	20907

Applications	ELISA
Applications Notes	This Mouse Syntaxin-1A (STX1A) ELISA Kit employs a two-site sandwich ELISA to quantitate STX1A in samples. An antibody specific for STX1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTX1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STX1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STX1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705436	KTE70276	48 T	$359		Add to Cart
BHE13705436	KTE70276	96 T	$619		Add to Cart
BHE13705436	KTE70276	96 Tx5	$2809		Add to Cart
BHE13705436	KTE70276	96 Tx50	$26209		Add to Cart

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