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Home / ELISA Kit

Mouse Syncoilin (SYNC) ELISA Kit

SKU : BHE13705444

Syncollin is a 13 kDa protein that is highly expressed in the exocrine pancreas. Syncollin normally exists as a doughnut-shaped homo-oligomer (quite probably a hexamer) in close association with the luminal surface of the zymogen granule membrane.
Functions in exocytosis in pancreatic acinar cells regulating the fusion of zymogen granules with each other. May have a pore-forming activity on membranes and regulate exocytosis in other exocrine tissues.
Syncollin is a small protein that is abundantly expressed in pancreatic acinar cells and that is tightly associated with the lumenal side of the zymogen granule membrane.

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Mfr.No.	KTE70284
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Syncoilin (SYNC) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNC.
Alternative Names	SYNC; MGC149625; MGC149626; SYNC1; SYNCOILIN; intermediate filament protein syncoilin; syncoilin; intermediate filament 1
Detection Method	Colorimetric
Kit Components	Mouse Syncoilin microplate Mouse Syncoilin standard Mouse Syncoilin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Syncoilin (SYNC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNC. No significant cross-reactivity or interference between Mouse SYNC and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Syncollin is a 13 kDa protein that is highly expressed in the exocrine pancreas. Syncollin normally exists as a doughnut-shaped homo-oligomer (quite probably a hexamer) in close association with the luminal surface of the zymogen granule membrane. Functions in exocytosis in pancreatic acinar cells regulating the fusion of zymogen granules with each other. May have a pore-forming activity on membranes and regulate exocytosis in other exocrine tissues. Syncollin is a small protein that is abundantly expressed in pancreatic acinar cells and that is tightly associated with the lumenal side of the zymogen granule membrane.
Notes	This Mouse Syncoilin (SYNC) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNC in samples. An antibody specific for SYNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	68828

Applications	ELISA
Applications Notes	This Mouse Syncoilin (SYNC) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNC in samples. An antibody specific for SYNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705444	KTE70284	48 T	$339		Add to Cart
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BHE13705444	KTE70284	96 Tx5	$2629		Add to Cart
BHE13705444	KTE70284	96 Tx50	$24569		Add to Cart

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