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Home / ELISA Kit

Mouse Synaptotagmin-like protein 5 (SYTL5) ELISA Kit

SKU : BHE13705475

The protein encoded by SYTL5 belongs to the synaptotagmin-like (Slp) protein family, which contains a unique homology domain at the N-terminus, referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of SYTL5 is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for SYTL5.

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Mfr.No.	KTE70315
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Synaptotagmin-like protein 5 (SYTL5) ELISA Kit employs a two-site sandwich ELISA to quantitate SYTL5.
Alternative Names	SYTL5; slp5; OTTHUMP00000031916; exophilin 9
Detection Method	Colorimetric
Kit Components	Mouse Synaptotagmin-like protein 5 microplate Mouse Synaptotagmin-like protein 5 standard Mouse Synaptotagmin-like protein 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Synaptotagmin-like protein 5 (SYTL5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYTL5. No significant cross-reactivity or interference between Mouse SYTL5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The protein encoded by SYTL5 belongs to the synaptotagmin-like (Slp) protein family, which contains a unique homology domain at the N-terminus, referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of SYTL5 is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for SYTL5.
Notes	This Mouse Synaptotagmin-like protein 5 (SYTL5) ELISA Kit employs a two-site sandwich ELISA to quantitate SYTL5 in samples. An antibody specific for SYTL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYTL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYTL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYTL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	236643

Applications	ELISA
Applications Notes	This Mouse Synaptotagmin-like protein 5 (SYTL5) ELISA Kit employs a two-site sandwich ELISA to quantitate SYTL5 in samples. An antibody specific for SYTL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYTL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYTL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYTL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705475	KTE70315	48 T	$339		Add to Cart
BHE13705475	KTE70315	96 T	$589		Add to Cart
BHE13705475	KTE70315	96 Tx5	$2629		Add to Cart
BHE13705475	KTE70315	96 Tx50	$24569		Add to Cart

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