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Home / ELISA Kit

Mouse Synaptotagmin-12 (SYT12) ELISA Kit

SKU : BHE13705457

SYT12 is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. Studies of the orthologous gene in rat have shown that the encoded protein selectively modulates spontaneous synaptic-vesicle exocytosis and may also be involved in regulating calcium independent secretion in nonneuronal cells. Alternative splicing results in multiple transcript variants. SYT12 has previously been referred to as synaptotagmin XI but has been renamed synaptotagmin XII to be standard with mouse and rat official nomenclature.

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Mfr.No.	KTE70297
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Synaptotagmin-12 (SYT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT12.
Alternative Names	SYT12; SRG1; SYT11;
Detection Method	Colorimetric
Kit Components	Mouse Synaptotagmin-12 microplate Mouse Synaptotagmin-12 standard Mouse Synaptotagmin-12 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Synaptotagmin-12 (SYT12) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYT12. No significant cross-reactivity or interference between Mouse SYT12 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SYT12 is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. Studies of the orthologous gene in rat have shown that the encoded protein selectively modulates spontaneous synaptic-vesicle exocytosis and may also be involved in regulating calcium independent secretion in nonneuronal cells. Alternative splicing results in multiple transcript variants. SYT12 has previously been referred to as synaptotagmin XI but has been renamed synaptotagmin XII to be standard with mouse and rat official nomenclature.
Notes	This Mouse Synaptotagmin-12 (SYT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT12 in samples. An antibody specific for SYT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	171180

Applications	ELISA
Applications Notes	This Mouse Synaptotagmin-12 (SYT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT12 in samples. An antibody specific for SYT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705457	KTE70297	48 T	$339		Add to Cart
BHE13705457	KTE70297	96 T	$589		Add to Cart
BHE13705457	KTE70297	96 Tx5	$2629		Add to Cart
BHE13705457	KTE70297	96 Tx50	$24569		Add to Cart

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