Mouse Super Oxidase Dimutase,SOD ELISA Kit

Mouse Super Oxidase Dimutase,SOD ELISA Kit

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Mfr.No.

CSB-E08556m

Sensitivity

5.4 pg/mL

Detection Range

5.4 pg/mL-4000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

superoxide dismutase 1, soluble, ALS, ALS1, IPOA, SOD, homodimer, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase, cystolic

Recovery

The recovery of human BK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	97	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	93
	Range %	90-95
1:200	Average %	103
	Range %	98-107
1:400	Average %	93
	Range %	86-97
1:800	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
20	2.586	2.566	2.576	2.467
10	2.224	2.305	2.265	2.156
5	1.711	1.681	1.696	1.587
2.5	1.085	1.077	1.081	0.972
1.25	0.624	0.630	0.627	0.518
0.625	0.379	0.368	0.374	0.265
0.312	0.243	0.232	0.238	0.129
0	0.110	0.108	0.109	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08228

Applications	ELISA
Research Area	SOD1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10506740	CSB-E08556m	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.