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Home / ELISA Kit

Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit

SKU : BHE13705440

Phenol sulfotransferase, or PST (EC 2.8.2.1), catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. PST is widely distributed in human and animal tissues that include the blood platelets. Human platelet PST exists in at least a thermolabile, or monoamine-metabolizing (SULT1A3, or STM), form and a thermostable, or phenol-metabolizing, form. Evidence that inheritance contributes to differences in levels of the 2 forms of the enzyme in whites was presented by Reveley et al. (1982), Price et al. (1988), and Van Loon and Weinshilboum (1984). Anderson and Jackson (1984) showed that the mean basal level of platelet thermostable PST activity in American blacks is significantly higher than the mean basal level in whites, whereas the average platelet thermolabile PST activity does not differ significantly in the 2 groups.

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Mfr.No.	KTE70280
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SULT1A1.
Alternative Names	SULT1A1; HAST1/HAST2; MGC131921; MGC5163; P-PST; PST; ST1A3; STP; STP1; TSPST1; aryl sulfotransferase; phenol-preferring phenol sulfotransferase1; phenol-sulfating phenol sulfotransferase; thermostable phenol s
Detection Method	Colorimetric
Kit Components	Mouse Sulfotransferase 1A1 microplate Mouse Sulfotransferase 1A1 standard Mouse Sulfotransferase 1A1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SULT1A1. No significant cross-reactivity or interference between Mouse SULT1A1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Phenol sulfotransferase, or PST (EC 2.8.2.1), catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. PST is widely distributed in human and animal tissues that include the blood platelets. Human platelet PST exists in at least a thermolabile, or monoamine-metabolizing (SULT1A3, or STM), form and a thermostable, or phenol-metabolizing, form. Evidence that inheritance contributes to differences in levels of the 2 forms of the enzyme in whites was presented by Reveley et al. (1982), Price et al. (1988), and Van Loon and Weinshilboum (1984). Anderson and Jackson (1984) showed that the mean basal level of platelet thermostable PST activity in American blacks is significantly higher than the mean basal level in whites, whereas the average platelet thermolabile PST activity does not differ significantly in the 2 groups.
Notes	This Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SULT1A1 in samples. An antibody specific for SULT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySULT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULT1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SULT1A1 in samples. An antibody specific for SULT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySULT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULT1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705440	KTE70280	48 T	$339		Add to Cart
BHE13705440	KTE70280	96 T	$589		Add to Cart
BHE13705440	KTE70280	96 Tx5	$2629		Add to Cart
BHE13705440	KTE70280	96 Tx50	$24569		Add to Cart

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