Mouse substance P receptor,SP-R ELISA Kit

Mouse substance P receptor,SP-R ELISA Kit

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Mfr.No.

CSB-E08362m

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tachykinin receptor 1, NK1R, NKIR, SPR, TAC1R, NK-1 receptor, neurokinin 1 receptor, tachykinin 1 receptor (substance P receptor, neurokinin 1 receptor), tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)

Recovery

The recovery of rat APP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	95	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat APP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	92
	Range %	88-99
1:4	Average %	100
	Range %	95-107
1:8	Average %	90
	Range %	86-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.557	2.520	2.539	2.429
500	1.961	1.876	1.919	1.809
250	1.197	1.204	1.201	1.091
125	0.748	0.809	0.779	0.669
62.5	0.436	0.446	0.441	0.331
31.2	0.278	0.259	0.269	0.159
15.6	0.206	0.209	0.208	0.098
0	0.112	0.107	0.110

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P30548

Applications	ELISA
Research Area	TACR1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.