Mouse Stromal cell derived factor 1beta,SDF-1beta ELISA kit

Mouse Stromal cell derived factor 1beta,SDF-1beta ELISA kit

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Mfr.No.

CSB-E04723m

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1), stromal cell derived factor 1beta(SDF-1beta/SDF1B), stromal cell derived factor 1 beta(SDF-1-beta/SDF1B)

Recovery

The recovery of human S100P spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	96	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S100P in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	81-89
1:10	Average %	89
	Range %	82-93
1:20	Average %	87
	Range %	82-90
1:40	Average %	93
	Range %	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.684	2.901	2.793	2.631
2500	1.963	1.999	1.981	1.819
1250	1.168	1.121	1.145	0.983
625	0.632	0.652	0.642	0.480
312.5	0.415	0.436	0.426	0.264
156	0.309	0.320	0.315	0.153
78	0.268	0.255	0.262	0.100
0	0.161	0.163	0.162

Species

Mus musculus

Sample Type

Serum, Cell culture supeatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P40224
Tissue Specificity	Highest expression levels detected in kidney, liver, spleen and muscle. Isoform Alpha is expressed ubiquitously but at varying levels, while isoform Beta displays tissue-specific expression, with expression detected in kidney, liver, heart, spleen and mus

Applications	ELISA
Research Area	CXCL12

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506733	CSB-E04723m	96T	$615	Add to Cart
BHE10506733	CSB-E04723m	5x96T	$2952	Add to Cart
BHE10506733	CSB-E04723m	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.