Stat3 ELISA Kit, Signal transducer and activator of transcription 3 ELISA Kit
Detection Method
Double Antibody
Recovery
Matrices listed below were spiked with certain level of Stat3 and the recovery rates were calculated by comparing the measured value to the expected amount of Stat3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-98
93
EDTA plasma(n=5)
85-105
94
heparin plasma(n=5)
85-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stat3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
89-102%
95-102%
EDTA plasma(n=5)
88-98%
85-94%
86-101%
heparin plasma(n=5)
85-100%
85-92%
89-97%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Stat3 ELISA Kit allows for the in vitro quantitative determination of Stat3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Mouse Stat3 (Signal transducer and activator of transcription 3) ELISA Kit