Mouse SPINT1/HAI-1 ELISA Kit PicoKine®

Mfr.No.

EK0772

Sensitivity

<12 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.007	0.084	0.165	0.304	0.585	0.93	1.636	2.225

Description

Mouse SPINT1/HAI-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Spint1 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Kunitz-type protease inhibitor 1;Hepatocyte growth factor activator inhibitor type 1;HAI-1;Spint1;Hai1;

Molecular Weight

56591 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-L507

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SPINT1

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	199	707	1860	185	728	1735
Standard deviation	14.72	41	119.04	16.4%1	48.1%3	140.53
CV (%)	7.4%	5.8%	6.4%	8.6%	6.2%	8.1%

Protein Function

Inhibitor of HGF activator. .

Background

Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alteative splicing results in multiple variants encoding different isoforms.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	199	180	196	188	190	7.39	3.8%
Sample 2	707	699	666	710	695	17.5	2.5%
Sample 3	1860	1696	2012	1755	1830	120	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20732
Gene Name	Spint1
UniProt ID	Q9R097
Protein Name	Kunitz-type protease inhibitor 1

Applications	ELISA
Research Area	Cell Biology,Protease Inhibitors,Proteolysis/Ubiquitin

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001067	EK0772	96 wells/kit	$499		Add to Cart

Mouse SPINT1/HAI-1 ELISA Kit PicoKine®

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