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Home / ELISA Kit

Mouse Speckle-type POZ protein (SPOP) ELISA Kit

SKU : BHE13705400

SPOP encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.The SPOP gene encodes a 374-amino acid polypeptide that contains a poxvirus and zinc finger (POZ) domain and an evolutionarily conserved N-terminal region.

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Mfr.No.	KTE70240
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Speckle-type POZ protein (SPOP) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOP.
Alternative Names	SPOP; TEF2;
Detection Method	Colorimetric
Kit Components	Mouse Speckle-type POZ protein microplate Mouse Speckle-type POZ protein standard Mouse Speckle-type POZ protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Speckle-type POZ protein (SPOP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPOP. No significant cross-reactivity or interference between Mouse SPOP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SPOP encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.The SPOP gene encodes a 374-amino acid polypeptide that contains a poxvirus and zinc finger (POZ) domain and an evolutionarily conserved N-terminal region.
Notes	This Mouse Speckle-type POZ protein (SPOP) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOP in samples. An antibody specific for SPOP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	20747

Applications	ELISA
Applications Notes	This Mouse Speckle-type POZ protein (SPOP) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOP in samples. An antibody specific for SPOP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705400	KTE70240	48 T	$339		Add to Cart
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