Mouse Sparc(SPARC) ELISA Kit

Mfr.No.

EM0457

Alternative Names

Sparc ELISA Kit, SPARC ELISA Kit, BM-40 ELISA Kit, Osteonectin ELISA Kit, Basement-membrane protein 40 ELISA Kit, BM-40 ELISA Kit, ONcysteine-rich protein ELISA Kit, osteonectin ELISA Kit, Osteonectin ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit, secreted protein ELISA Kit, acidic ELISA Kit, cysteine-rich ELISA Kit, osteonectin ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

3.125-200ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Sparc and the recovery rates were calculated by comparing the measured value to the expected amount of Sparc in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-93	91
EDTA plasma(n=5)	85-103	94
heparin plasma(n=5)	87-101	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sparc and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	98-105%	94-105%	88-94%
EDTA plasma(n=5)	84-93%	84-98%	82-98%
heparin plasma(n=5)	83-98%	82-96%	89-94%

Sensitivity

< 1.875ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Sparc/SPARC/BM-40/Osteonectin/Basement-membrane protein 40/BM-40/ONcysteine-rich protein/osteonectin/Osteonectin/Secreted protein acidic and rich in cysteine/secreted protein, acidic, cysteine-rich(osteonectin)

Notes

For Research Use Only

UniProt ID	P07214
Antigen	SPARC

Applications	ELISA
Applications Notes	Sparc ELISA Kit allows for the in vitro quantitative determination of Sparc concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10801361	EM0457	96T	$400		Add to Cart

Mouse Sparc (SPARC) ELISA Kit

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