Mouse SPARC-like protein 1(SPARCL1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL022487MO

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

SPARC-like 1 (hevin), PIG33, SC1, SPARC-like 1, SPARC-like 1 (mast9, hevin), hevin, mast9, proliferation-inducing protein 33

Recovery

The recovery of pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	103	98-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	92
	Range %	88-95
1:400	Average %	93
	Range %	90-97
1:800	Average %	88
	Range %	85-95
1:1600	Average %	95
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P70663

Applications	ELISA
Research Area	SPARCL1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10506721	CSB-EL022487MO	96T	$695	Add to Cart
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BHE10506721	CSB-EL022487MO	10x96T	$6602.50	Add to Cart