Matrices listed below were spiked with certain level of SOST and the recovery rates were calculated by comparing the measured value to the expected amount of SOST in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
92
EDTA plasma(n=5)
86-103
95
heparin plasma(n=5)
86-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SOST and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-102%
88-95%
90-105%
EDTA plasma(n=5)
91-100%
87-99%
93-101%
heparin plasma(n=5)
88-99%
87-97%
85-99%
Sensitivity
< 7.5pg/ml
Notes
For Research Use Only
UniProt ID
Q99P68
Applications
ELISA
Applications Notes
SOST ELISA Kit allows for the in vitro quantitative determination of SOST concentrations in serum, plasma, tissue homogenates and other biological fluids.