Mouse Sonic hedgehog protein(SHH) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL021266MO

Sensitivity

7 pg/mL

Detection Range

28 pg/mL-1800 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

sonic hedgehog homolog (Drosophila), HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, sonic hedgehog

Recovery

The recovery of bovine IFN-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-101
1:2	Average %	91
	Range %	85-98
1:4	Average %	95
	Range %	90-103
1:8	Average %	96
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q62226

Applications	ELISA
Research Area	SHH

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506717	CSB-EL021266MO	96T	$695	Add to Cart
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BHE10506717	CSB-EL021266MO	10x96T	$6602.50	Add to Cart