Mouse soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA KIT

Mouse soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA KIT

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Mfr.No.

CSB-E07371m

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor (ligand) superfamily, member 10, APO2L, Apo-2L, CD253, TL2, TRAIL, Apo-2 ligand, TNF-related apoptosis inducing ligand TRAIL, tumor necrosis factor (ligand) family, member 10, tumor necrosis factor apoptosis-inducing ligand splice var

Recovery

The recovery of human IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	93	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-95
1:2	Average %	93
	Range %	88-99
1:4	Average %	92
	Range %	87-100
1:8	Average %	95
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.058	2.011	2.035	1.931
500	1.664	1.547	1.606	1.502
250	1.188	1.149	1.169	1.065
125	0.739	0.704	0.722	0.618
62.5	0.561	0.521	0.541	0.437
31.2	0.375	0.354	0.365	0.261
15.6	0.219	0.211	0.215	0.111
0	0.105	0.103	0.104	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P50592

Applications	ELISA
Research Area	TNFSF10

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.