Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

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Mfr.No.

CSB-E11155m

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

myosin, heavy chain 1, skeletal muscle, adult, MGC133384, MYHSA1, MYHa, MyHC-2X/D, myosin heavy chain IIx/d, myosin, heavy polypeptide 1, skeletal muscle, adult

Recovery

The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-100
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	94
	Range %	89-100
1:4	Average %	94
	Range %	87-102
1:8	Average %	95
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.202	0.211	0.207
250	0.350	0.349	0.350
80	0.600	0.594	0.597
20	1.029	1.018	1.024
5	1.326	1.345	1.336
0	2.102	2.130	2.116

Species

Mus musculus

Sample Type

Serum, Cell culture supeatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q5SX40

Applications	ELISA
Research Area	MYH1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10506706	CSB-E11155m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.