Mouse Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

Mfr.No.

CSB-E04717m

Sensitivity

0.117 ng/mL

Detection Range

0.47 ng/mL-30 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD40 ligand, RP3-527F8.3, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 antigen ligand, T-B cell-activating molecule, TNF-related activation protein, tumor necrosis factor (ligand) superfa

Recovery

The recovery of human TPI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-107
EDTA plasma (n=4)	92	85-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TPI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	106
	Range %	100-112
1:2	Average %	95
	Range %	92-99
1:4	Average %	90
	Range %	86-96
1:8	Average %	93
	Range %	90-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1800	2.377	2.433	2.405	2.308
900	1.646	1.774	1.710	1.613
450	1.332	1.285	1.309	1.212
225	0.857	0.922	0.890	0.793
112.5	0.465	0.470	0.468	0.371
56.25	0.322	0.300	0.311	0.214
28.1	0.203	0.204	0.204	0.107
0	0.094	0.099	0.097	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P27548

Applications	ELISA
Research Area	CD40LG

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506698	CSB-E04717m	96T	$595	Add to Cart
BHE10506698	CSB-E04717m	5x96T	$2856	Add to Cart
BHE10506698	CSB-E04717m	10x96T	$5652.50	Add to Cart

Mouse Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

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