Mouse SOD(Super Oxidase Dimutase) ELISA Kit

Mfr.No.

ELK8749

Alternative Names

Super Oxidase Dimutase

Reactivity

Mouse

Detection Range

3.13-200 U/L

Sample Type

Tissue homogenates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant SOD and the recovery rates were calculated by comparing the measured value to the expected amount of SOD in samples.

Matrix	Recovery range	Average
serum(n=5)	94-107%	100%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	94-105%	98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SOD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	87-101%	89-99%	85-95%
EDTA plasma(n=5)	96-101%	85-92%	79-96%	89-101%
Heparin plasma(n=5)	95-102%	93-101%	97-105%	95-103%

Typical Data

Concentration (U/L)	OD	Corrected OD
200.00	2.027	1.942
100.00	1.653	1.568
50.00	1.143	1.058
25.00	0.826	0.741
12.50	0.596	0.511
6.25	0.366	0.281
3.13	0.213	0.128
0.00	0.085	0.000

Sensitivity

1.39 U/L

Limit Of Detection

200 U/L

UniProt ID	P08228
Antigen	SODELISAKit
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SOD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SOD in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;Tumor immunity;Infection immunity;Hepatology;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15205760	ELK8749	48T	$320		Add to Cart
BHE15205760	ELK8749	96T	$458		Add to Cart

Mouse SOD(Super Oxidase Dimutase) ELISA Kit

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