Mouse SMOC1 ELISA Kit PicoKine®

Mfr.No.

EK1936

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.104	0.148	0.189	0.261	0.456	0.762	1.223	1.982

Description

Mouse SMOC1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Smoc1 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

SPARC-related modular calcium-binding protein 1; SPARC-related gene protein; Secreted modular calcium-binding protein 1; SMOC-1; Smoc1; Srg

Immunogen

Expression system for standard: NS0; Immunogen sequence: H26-V463

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SMOC1

Subcellular Localization

Basement membrane

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	162	656	1364	168	695	1436
Standard deviation	8.42	41.33	65.47	8.23	51.43	76.11
CV (%)	5.2%	6.3%	4.8%	4.9%	7.4%	5.3%

Protein Function

Probable regulator of osteoblast differentiation. Plays essential roles in both eye and limb development.

Background

This gene is mapped to 12; 12 D1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	162	172	180	169	171	3.07	1.8%
Sample 2	656	652	674	688	668	24.7	3.7%
Sample 3	1364	1412	1531	1296	1401	81.24	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	64075
Gene Name	Smoc1
UniProt ID	Q8BLY1
Tissue Specificity	Widely expressed in many tissues with a strongest signal in ovary.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001453	EK1936	96 wells/kit	$750		Add to Cart

Mouse SMOC1 ELISA Kit PicoKine®

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