Mouse Sirt1(NAD-dependent deacetylase sirtuin-1) ELISA Kit

Mfr.No.

EM0773

Alternative Names

Sirt1 ELISA Kit, NAD-dependent deacetylase sirtuin-1 ELISA Kit, SIRT1 ELISA Kit, SIR2-like protein 1 ELISA Kit, SIR2 ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

31.25-2000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Sirt1 and the recovery rates were calculated by comparing the measured value to the expected amount of Sirt1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-101	93
EDTA plasma(n=5)	87-101	93
heparin plasma(n=5)	86-102	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sirt1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	92-98%	89-104%	87-102%
EDTA plasma(n=5)	90-101%	84-97%	82-101%
heparin plasma(n=5)	80-97%	80-99%	81-97%

Sensitivity

< 18.75pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Sirt1O/NAD-dependent deacetylase sirtuin-1(SIRT1)/SIR2-like protein 1(SIR2)

Notes

For Research Use Only

UniProt ID	Q923E4
Antigen	Sirt1O

Applications	ELISA
Applications Notes	Sirt1 ELISA Kit allows for the in vitro quantitative determination of Sirt1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10805431	EM0773	96T	$400		Add to Cart

Mouse Sirt1(NAD-dependent deacetylase sirtuin-1) ELISA Kit

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