Mouse sex hormone-binding globulin,SHBG ELISA Kit

Mfr.No.

CSB-E08233m

Sensitivity

0.33 ng/mL

Detection Range

1.3 ng/mL-50 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

sex hormone-binding globulin, ABP, MGC126834, MGC138391, SBP, TEBG, androgen binding protein, sex steroid-binding protein, testis-specific androgen-binding protein, testosterone-binding beta-globulin, testosterone-estradiol-binding

Recovery

The recovery of rat APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-94
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	96
	Range %	92-100
1:400	Average %	90
	Range %	85-96
1:800	Average %	90
	Range %	88-94
1:1600	Average %	100
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.454	2.460	2.457	2.319
50	2.045	2.032	2.039	1.901
25	1.411	1.482	1.447	1.309
12.5	0.831	0.901	0.866	0.728
6.25	0.631	0.625	0.628	0.490
3.12	0.379	0.408	0.394	0.256
1.56	0.277	0.272	0.275	0.137
0	0.135	0.140	0.138	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P97497

Applications	ELISA
Research Area	SHBG

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506680	CSB-E08233m	96T	$835	Add to Cart
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BHE10506680	CSB-E08233m	10x96T	$7932.50	Add to Cart

Mouse sex hormone-binding globulin,SHBG ELISA Kit

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