Mouse Semaphorin-3A - SEMA3A ELISA Kit

Mfr.No.

E2446Mo

Sensitivity

5.15ng/L

Detection Range

10-2000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

SEMA 3A;Sema D;Sema III;SEMA3A;Semaphorin III;Semaphorin-3A;Semaphorin-D

Molecular Weight

88813

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2446Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	602.7	20.85	3.5
Intra-Assay	2	18	189.8	11.75	6.2
Intra-Assay	3	18	264.3	10.91	4.1

Background

Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).

Protocol

These standard curves of E2446Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.551	2.581	2.543
	2.611
600ng/L	1.565	1.562	1.524
	1.559
300ng/L	0.723	0.77	0.733
	0.818
150ng/L	0.441	0.483	0.446
	0.526
75ng/L	0.258	0.258	0.22
	0.258
0ng/L	0.035	0.038	0
	0.04

Gene ID	20346
UniProt ID	O08665

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110257	E2446Mo	48T	$320		Add to Cart
BHE12110257	E2446Mo	96T	$458		Add to Cart

Mouse Semaphorin-3A - SEMA3A ELISA Kit

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