Mouse SECTM1A ELISA Kit PicoKine®

Mfr.No.

EK1853

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.016	0.233	0.420	0.655	1.102	1.653	1.934	2.383

Description

Mouse SECTM1A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sectm1a in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Secreted and transmembrane protein 1A; Sectm1a

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q28-T165

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SECTM1A

Subcellular Localization

Secreted. Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	19	74	299	28	89	354
Standard deviation	0.99	3.92	16.15	1.71	5.25	24.07
CV (%)	5.2%	5.3%	5.4%	6.1%	5.9%	6.8%

Background

Secreted and transmembrane 1A is a protein that in humans is encoded by the SECTM1A gene. It is mapped to 11; 11 E2. This gene encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like patte and thought to be involved in hematopoietic and/or immune system processes.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	19	19	18	19	18	0.43	2.3%
Sample 2	74	83	76	88	80	5.58	6.9%
Sample 3	299	344	333	346	330	18.84	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	209588
Gene Name	Sectm1a
UniProt ID	Q921W8

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001618	EK1853	96 wells/kit	$750		Add to Cart

Mouse SECTM1A ELISA Kit PicoKine®

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