Matrices listed below were spiked with certain level of SCN9A and the recovery rates were calculated by comparing the measured value to the expected amount of SCN9A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
93
EDTA plasma(n=5)
86-102
96
heparin plasma(n=5)
85-105
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SCN9A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-97%
86-105%
85-103%
EDTA plasma(n=5)
84-97%
84-97%
84-98%
heparin plasma(n=5)
83-99%
82-99%
88-100%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q62205
Applications
ELISA
Applications Notes
SCN9AELISA Kit allows for the in vitro quantitative determination of SCN9A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse SCN9A(Sodium channel protein)ELISA Kit