Mouse Sclerostin/SOST ELISA Kit PicoKine®

Mfr.No.

EK1179

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.055	0.101	0.138	0.201	0.426	0.712	1.466	2.085

Description

Mouse Sclerostin/SOST ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sost in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Sclerostin;Sost;

Molecular Weight

23443 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-Y211

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Sclerostin

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	30	190	438	28	192	398
Standard deviation	2.04	13.49	22.77	2.24	15.55	28.05
CV (%)	6.8%	7.1%	5.2%	8%	8.1%	7.1%

Protein Function

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .

Background

Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	30	31	32	30	30	0.82	2.7%
Sample 2	190	179	179	181	182	4.54	2.4%
Sample 3	438	503	469	495	476	25.41	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	74499
Gene Name	SOST
UniProt ID	Q99P68
Protein Name	Sclerostin
Sequence	Belongs to the sclerostin family.

Applications	ELISA
Research Area	Cytoskeleton/ECM,Developmental Biology,Extracellular Matrix,Growth Factors/Hormones,Metabolism,Organogenesis,Secreted,Signal Transduction,Signaling Pathways,Skeletal Development,Stem Cells,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000801	EK1179	96 wells/kit	$399		Add to Cart

Mouse Sclerostin/SOST ELISA Kit PicoKine®

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