Matrices listed below were spiked with certain level of SCFR and the recovery rates were calculated by comparing the measured value to the expected amount of SCFR in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-97
93
EDTA plasma(n=5)
85-94
90
heparin plasma(n=5)
85-100
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SCFR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SCFR ELISA Kit allows for the in vitro quantitative determination of SCFR concentrations in serum, plasma, tissue homogenates and other biological fluids.