Mouse Scavenger Receptor Cysteine-rich Type 1 Protein M130

Mfr.No.

E2439Mo

Sensitivity

9.61ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CD 163;CD antigen CD163;CD163;CD163v2;CD163v3;Scavenger receptor cysteine-rich type 1 protein M130;sCD163;Soluble CD163

Molecular Weight

120921

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2439Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1309.32	65.71	5.02
Intra-Assay	2	18	210.84	18.46	8.76
Intra-Assay	3	18	363.12	19.58	5.39

Background

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity). After shedding, the soluble form (sCD163) may play an anti-inflammatory role.

Protocol

These standard curves of E2439Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.236	2.289	2.243
	2.342
1200ng/L	1.674	1.678	1.632
	1.681
600ng/L	1.056	1.034	0.988
	1.011
300ng/L	0.653	0.671	0.625
	0.689
150ng/L	0.451	0.47	0.424
	0.489
0ng/L	0.049	0.046	0
	0.043

Gene ID	93671
UniProt ID	Q2VLH6

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110250	E2439Mo	48T	$320		Add to Cart
BHE12110250	E2439Mo	96T	$458		Add to Cart

Mouse Scavenger Receptor Cysteine-rich Type 1 Protein M130 - CD163 ELISA Kit

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