Mouse SATB2(Special AT-rich sequence-binding protein 2) ELISA Kit

Mfr.No.

ELK9046

Sensitivity

13.2 pg/mL

Detection Range

31.25-2000 pg/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

DNA-binding protein SATB2; SATB2; KIAA1034; Special AT-rich sequence-binding protein 2

Recovery

Matrices listed below were spiked with certain level of recombinant SATB2 and the recovery rates were calculated by comparing the measured value to the expected amount of SATB2 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	85-97%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SATB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	84-98%	95-105%	88-102%	85-97%
EDTA plasma(n=5)	95-107%	92-103%	81-99%	93-102%
Heparin plasma(n=5)	87-98%	83-97%	91-98%	83-101%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.126	2.034
1000.00	1.634	1.542
500.00	1.116	1.024
250.00	0.896	0.804
125.00	0.543	0.451
62.50	0.297	0.205
31.25	0.256	0.164
0.00	0.092	0.000

Limit Of Detection

2000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SATB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SATB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SATB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SATB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	Q8VI24

Applications	ELISA
Research Area	Signal transduction;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15205727	ELK9046	48T	$412		Add to Cart
BHE15205727	ELK9046	96T	$588		Add to Cart

Mouse SATB2(Special AT-rich sequence-binding protein 2) ELISA Kit

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