Serum amyloid A-3 protein ELISA Kit, saa3 ELISA Kit
Reactivity
Mouse
Detection Method
Double Antibody
Detection Range
15.625-1000pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of saa3 and the recovery rates were calculated by comparing the measured value to the expected amount of saa3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-103
98
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
88-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of saa3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-103%
87-103%
87-95%
EDTA plasma(n=5)
82-101%
84-97%
83-94%
heparin plasma(n=5)
81-99%
83-100%
81-94%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P04918
Applications
ELISA
Applications Notes
saa3 allows for the in vitro quantitative determination of saa3 concentrations in serum, plasma, tissue homogenates and other biological fluids.