Mouse SAA/SAA1 ELISA Kit PicoKine®

Mfr.No.

EK1190

Sensitivity

<20 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.079	0.108	0.215	0.437	0.535	0.990	1.423	1.872

Description

Mouse SAA/SAA1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Saa1 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serum amyloid A-1 protein;Saa1;

Molecular Weight

13770 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G20-Y122

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SAA

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	270	1129	2924	194	1044	3393
Standard deviation	9.26	70.9	166.67	9.72	52.93	245.81
CV (%)	3.4%	6.3%	5.7%	5%	5.1%	7.5%

Protein Function

Major acute phase protein. .

Background

Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	270	207	211	200	222	16.23	7.3%
Sample 2	1129	1020	856	655	915	32.12	3.5%
Sample 3	2924	4991	2925	4112	3738	240.73	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	111345
Gene Name	SAA1
UniProt ID	P05366
Protein Name	Serum amyloid A-1 protein
Tissue Specificity	Detected in blood plasma (at protein level). Detected in liver. .

Applications	ELISA
Research Area	Cardiovascular,Complement,Cytokines,G Protein Signaling,Immunology,Innate Immunity,Lipids/Lipoproteins,Macrophage/Inflammation,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000810	EK1190	96 wells/kit	$499		Add to Cart

Mouse SAA/SAA1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse SAA/SAA1 ELISA Kit PicoKine®

Rate it now..!!