Mouse S100A9(S100 Calcium Binding Protein A9) ELISA Kit

Mfr.No.

ELK3285

Sensitivity

0.059 ng/mL

Detection Range

0.16-10 ng/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

Recovery

Matrices listed below were spiked with certain level of recombinant S100A9 and the recovery rates were calculated by comparing the measured value to the expected amount of S100A9 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	83-98%	91%
Heparin plasma(n=5)	91-99%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100A9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-96%	87-101%	89-102%	85-98%
EDTA plasma(n=5)	86-101%	86-101%	87-96%	92-105%
Heparin plasma(n=5)	89-102%	92-101%	88-102%	87-102%

Typical Data

<table> <tbody><tr> <th>Concentration (ng/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>10.00</td> <td>2.035</td> <td>1.950</td> </tr> <tr> <td>5.00</td> <td>1.703</td> <td>1.618</td> </tr> <tr> <td>2.50</td> <td>1.157</td> <td>1.072</td> </tr> <tr> <td>1.25</td> <td>0.895</td> <td>0.810</td> </tr> <tr> <td>0.63</td> <td>0.585</td> <td>0.500</td> </tr> <tr> <td>0.32</td> <td>0.363</td> <td>0.278</td> </tr> <tr> <td>0.16</td> <td>0.257</td> <td>0.172</td> </tr> <tr> <td>0.00</td> <td>0.085</td> <td>0.000</td> </tr> </tbody></table>

Limit Of Detection

10 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100A9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100A9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3285-1.pdf" target="_blank">Datasheet</a>

UniProt ID	P31725

Applications	ELISA
Research Area	Tumor immunity;Infection immunity;Rheumatology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206765	ELK3285	48T	$320		Add to Cart
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Mouse S100A9(S100 Calcium Binding Protein A9) ELISA Kit

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