Mouse S100A8/Calgranulin A ELISA Kit PicoKine®

Mfr.No.

EK1150

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.077	0.116	0.208	0.372	0.514	0.835	1.204	1.897

Description

Mouse S100A8/Calgranulin A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse S100a8 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Protein S100-A8;Calgranulin-A;Chemotactic cytokine CP-10;Leukocyte L1 complex light chain;Migration inhibitory factor-related protein 8;MRP-8;p8;Pro-inflammatory S100 cytokine;S100 calcium-binding protein A8;S100a8;Caga, Mrp8;

Molecular Weight

10295 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P2-E89

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse S100A8

Subcellular Localization

Secreted . Cytoplasm . Cytoplasm, cytoskeleton . Cell membrane ; Peripheral membrane protein . Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alteative pathway.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	276	1214	4039	294	1228	4594
Standard deviation	11.59	52.20	165.60	19.40	83.50	326.17
CV (%)	4.2%	4.3%	4.1%	6.6%	6.8%	7.1%

Protein Function

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by ly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular patte (DAMP) molecule and stimulates innate immune cells via binding to patte recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn (2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to selective inflammatory stimulus-dependent S- nitrosylation of multiple targets such as GAPDH, ANXA5, EZR, MSN and VIM by recognizing a (IL)-x-C-x-x-(DE) motif; S100A8 seems to contribute to S-nitrosylation site selectivity (By similarity). .

Background

S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	276	253	272	279	270	10.12	3.7%
Sample 2	1214	1248	1303	1250	1253	31.83	2.5%
Sample 3	4039	3861	4494	4141	4133	230.86	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20201
Gene Name	S100a8
UniProt ID	P27005
Protein Name	Protein S100-A8

Applications	ELISA
Research Area	Cancer,Cell Type Marker,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001014	EK1150	96 wells/kit	$399		Add to Cart

Mouse S100A8/Calgranulin A ELISA Kit PicoKine®

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