Mouse RPS6KA3(Ribosomal Protein S6 Kinase Alpha 3) ELISA Kit

Mfr.No.

ELK0489

Alternative Names

"90 kDa ribosomal protein S6 kinase 3 (p90-RSK 3; p90RSK3) MAP kinase-activated protein kinase 1b (MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b) Ribosomal S6 kinase 2 (RSK-2) pp90RSK2"

Reactivity

Mouse

Detection Range

15.63-1000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant RPS6KA3 and the recovery rates were calculated by comparing the measured value to the expected amount of RPS6KA3 in samples.

Matrix	Recovery range	Average
serum(n=5)	94-107%	100%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	82-95%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RPS6KA3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-93%	91-105%	87-99%	99-105%
EDTA plasma(n=5)	86-95%	91-106%	87-100%	95-101%
Heparin plasma(n=5)	87-98%	92-105%	95-107%	82-96%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.266	2.158
500.00	1.694	1.586
250.00	1.196	1.088
125.00	0.821	0.713
62.50	0.509	0.401
31.25	0.364	0.256
15.63	0.223	0.115
0.00	0.108	0.000

Sensitivity

6.81 pg/mL

Limit Of Detection

1000 pg/mL

Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RPS6KA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RPS6KA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RPS6KA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RPS6KA3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208764	ELK0489	48T	$320		Add to Cart
BHE15208764	ELK0489	96T	$458		Add to Cart

Mouse RPS6KA3(Ribosomal Protein S6 Kinase Alpha 3) ELISA Kit

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