Mouse Roundabout Homolog 4 - ROBO4 ELISA Kit

Mfr.No.

E2433Mo

Alternative Names

ROBO 4;ROBO4;Roundabout homolog 4

Reactivity

Mouse

Detection Range

0.06-18ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

108499

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.026ng/mL

Background

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function (By similarity).

Gene ID	74144
UniProt ID	Q8C310
Antigen	ROBO4

Research Area

Cancer

Protocol

These standard curves of E2433Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
9.6ng/mL	2.145	2.256	2.21
	2.367
4.8ng/mL	1.343	1.256	1.209
	1.169
2.4ng/mL	0.633	0.717	0.67
	0.8
1.2ng/mL	0.398	0.458	0.411
	0.518
0.6ng/mL	0.2	0.185	0.139
	0.171
0ng/mL	0.041	0.047	0
	0.053

Precision

We measured random samples of E2433Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.3	0.17	5.1
Intra-Assay	2	18	4.9	0.17	3.4
Intra-Assay	3	18	1.9	0.13	6.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110244	E2433Mo	48T	$320		Add to Cart
BHE12110244	E2433Mo	96T	$458		Add to Cart

Mouse Roundabout Homolog 4 - ROBO4 ELISA Kit

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