Matrices listed below were spiked with certain level of Reo-3 and the recovery rates were calculated by comparing the measured value to the expected amount of Reo-3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
98
EDTA plasma(n=5)
87-101
93
heparin plasma(n=5)
86-104
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Reo-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-101%
85-95%
92-102%
EDTA plasma(n=5)
82-100%
96-101%
87-99%
heparin plasma(n=5)
80-96%
83-96%
80-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Reovirus type 3 ELISA Kit allows for the in vitro quantitative determination of Reo-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.