Mouse R-Spondin-4 - RSPO4 ELISA Kit

Mfr.No.

E2437Mo

Alternative Names

A730099F22;A930029K19Rik;Cristin-4;Cysteine-rich and single thrombospondin domain-containing protein 4;mCristin-4;Roof plate-specific spondin-4;RSPO4;R-spondin-4

Reactivity

Mouse

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

25866

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.86ng/L

Background

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.

Gene ID	228770
UniProt ID	Q8BJ73
Antigen	RSPO4

Research Area

Others

Protocol

These standard curves of E2437Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.648	2.524	2.475
	2.4
1200ng/L	1.366	1.464	1.415
	1.562
600ng/L	0.854	0.812	0.763
	0.771
300ng/L	0.428	0.48	0.43
	0.531
150ng/L	0.271	0.265	0.216
	0.26
0ng/L	0.055	0.049	0
	0.044

Precision

We measured random samples of E2437Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1251.86	39.71	3.17
Intra-Assay	2	18	667.12	32.92	4.93
Intra-Assay	3	18	465.6	30.17	6.48

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110248	E2437Mo	48T	$320		Add to Cart
BHE12110248	E2437Mo	96T	$458		Add to Cart

Mouse R-Spondin-4 - RSPO4 ELISA Kit

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