Matrices listed below were spiked with certain level of Pycard and the recovery rates were calculated by comparing the measured value to the expected amount of Pycard in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-102
97
EDTA plasma(n=5)
85-102
91
heparin plasma(n=5)
85-100
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pycard and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
85-103%
86-104%
EDTA plasma(n=5)
86-99%
82-101%
83-101%
heparin plasma(n=5)
80-98%
88-100%
80-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9EPB4
Antigen
Pycard
Applications
ELISA
Applications Notes
Pycard ELISA Kit allows for the in vitro quantitative determination of Pycard concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Pycard(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit