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Home / ELISA Kit

Mouse Putative trace amine-associated receptor 3 (TAAR3) ELISA Kit

SKU : BHE13705483

Trace amine-associated receptors (TAARs), sometimes referred to as trace amine receptors (TAs or TARs), are a class of G protein-coupled receptors that were discovered in 2001.TAAR1, the first of six functional human TAARs, has gained considerable interest in academic and proprietary pharmaceutical research due to its unique role as the endogenous receptor for trace amines which are trace metabolic derivatives of phenylalanine and tryptophan and related psychostimulants, particularly amphetamine and methamphetamine. In 2004, it was shown that mammalian TAAR1 is also a receptor for thyronamines, which are decarboxylated and deiodinated metabolites of thyroid hormones.TAAR2TAAR9 function as olfactory receptors for volatile amines in vertebrates.

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Mfr.No.	KTE70323
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Putative trace amine-associated receptor 3 (TAAR3) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR3.
Alternative Names	TAAR3; GPR57; GPR58; TAAR2;
Detection Method	Colorimetric
Kit Components	Mouse Putative trace amine-associated receptor 3 microplate Mouse Putative trace amine-associated receptor 3 standard Mouse Putative trace amine-associated receptor 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Putative trace amine-associated receptor 3 (TAAR3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAAR3. No significant cross-reactivity or interference between Mouse TAAR3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Trace amine-associated receptors (TAARs), sometimes referred to as trace amine receptors (TAs or TARs), are a class of G protein-coupled receptors that were discovered in 2001.TAAR1, the first of six functional human TAARs, has gained considerable interest in academic and proprietary pharmaceutical research due to its unique role as the endogenous receptor for trace amines which are trace metabolic derivatives of phenylalanine and tryptophan and related psychostimulants, particularly amphetamine and methamphetamine. In 2004, it was shown that mammalian TAAR1 is also a receptor for thyronamines, which are decarboxylated and deiodinated metabolites of thyroid hormones.TAAR2TAAR9 function as olfactory receptors for volatile amines in vertebrates.
Notes	This Mouse Putative trace amine-associated receptor 3 (TAAR3) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR3 in samples. An antibody specific for TAAR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAAR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	493809

Applications	ELISA
Applications Notes	This Mouse Putative trace amine-associated receptor 3 (TAAR3) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR3 in samples. An antibody specific for TAAR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAAR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13705483	KTE70323	48 T	$339		Add to Cart
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