Mouse Pulmonary Surfatcant-associated Protein B

Mfr.No.

E1259Mo

Sensitivity

0.12ng/mL

Detection Range

0.2-60ng/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Pulmonary Surfatcant-associated Protein B (also known as SP-B) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Pulmonary surfactant-associated protein B;Pulmonary surfactant-associated proteolipid SPL(Phe;SFTPB;SP-B

Molecular Weight

41728

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1259Mo within the same batch/lot to ensure the consistency of the kits' performances.

Background

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Protocol

These standard curves of E1259Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/mL	2.217	2.192	2.148
	2.167
16ng/mL	1.678	1.654	1.611
	1.631
8ng/mL	1.09	1.075	1.032
	1.061
4ng/mL	0.78	0.768	0.725
	0.756
2ng/mL	0.439	0.447	0.404
	0.455
0ng/mL	0.057	0.043	0
	0.03

Gene ID	20388
UniProt ID	P50405

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109124	E1259Mo	48T	$320		Add to Cart
BHE12109124	E1259Mo	96T	$458		Add to Cart

Mouse Pulmonary Surfatcant-associated Protein B - SP-B ELISA Kit

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