Mouse pulmonary surfactant-associated protein D (SP-D) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12640m

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

pulmonary surfactant-associated protein D (SP-D)

Recovery

The recovery of rat GP-?b?a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	98	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GP-?b?a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	89
	Range %	82-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	90
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P50404

Applications	ELISA
Research Area	SP-D

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dietary lactosucrose suppresses influenza A (H1N1) virus infection in mice E KISHINO. et al,Bioscience of Microbiota, Food and Health,Vol. 34 (2015 ), No. 4 pp. 67-76).

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