Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit

Mfr.No.

CSB-E12638m

Sensitivity

0.11 ng/mL

Detection Range

0.45 ng/mL-30 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

surfactant protein B, PSP-B, SFTB3, SFTP3, SMDP1, SP-B, OTTHUMP00000203253, Pulmonary surfactant-associated protein B, 18kD, surfactant, pulmonary-associated protein B

Recovery

The recovery of rabbit IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	87-105
1:2000	Average %	92
	Range %	85-100
1:4000	Average %	93
	Range %	89-100
1:8000	Average %	94
	Range %	85-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
4000	0.225	0.218	0.222
1000	0.351	0.345	0.348
250	0.623	0.617	0.62
62.5	1.127	1.139	1.133
15.6	2.226	2.241	2.234
0	2.911	2.984	2.948

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P50405

Applications	ELISA
Research Area	SFTPB

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506582	CSB-E12638m	96T	$695	Add to Cart
BHE10506582	CSB-E12638m	5x96T	$3336	Add to Cart
BHE10506582	CSB-E12638m	10x96T	$6602.50	Add to Cart

Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit

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