Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

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Mfr.No.

CSB-E08685m

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

surfactant protein A1, AC068139.6, MGC133365, PSAP, PSPA, SFTP1, SFTPA1, pulmonary surfactant apoprotein, surfactant, pulmonary-associated protein A1, surfactant, pulmonary-associated protein A1B

Recovery

The recovery of human IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5000	Average %	86
	Range %	80-94
1:10000	Average %	97
	Range %	91-105
1:20000	Average %	95
	Range %	91-102
1:40000	Average %	94
	Range %	86-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.753	2.769	2.761	2.641
150	2.404	2.428	2.416	2.296
75	1.905	1.911	1.908	1.788
37.5	1.281	1.293	1.287	1.167
18.75	0.726	0.729	0.728	0.608
9.37	0.465	0.472	0.469	0.349
4.68	0.288	0.294	0.291	0.171
0	0.121	0.119	0.120	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P35242

Applications	ELISA
Research Area	SFTPA1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506581	CSB-E08685m	96T	$695	Add to Cart
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BHE10506581	CSB-E08685m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.