Mouse Protein Wnt-5a(WNT5A) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL026138MO

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

wingless-type MMTV integration site family, member 5A, hWNT5A, WNT-5A protein

Recovery

The recovery of human HEXIM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	82-98
EDTA plasma (n=4)	97	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HEXIM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	97
	Range %	90-105
1:4	Average %	99
	Range %	91-110
1:8	Average %	93
	Range %	80-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P22725

Applications	ELISA
Research Area	WNT5A

Storage	Store at 2-8°C. Please refer to protocol.

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