Mouse Protein Wnt-10B - WNT10B ELISA Kit

Mfr.No.

E2397Mo

Sensitivity

2.15ng/L

Detection Range

5-1000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Protein Wnt-10b;Protein Wnt-12;Wnt1;WNT10B;Wnt12

Molecular Weight

43119

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2397Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	439.42	8.81	2.01
Intra-Assay	2	18	372.16	15.91	4.27
Intra-Assay	3	18	160.7	8.39	5.22

Background

Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.

Protocol

These standard curves of E2397Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.152	2.032	1.982
	1.911
320ng/L	0.963	1.008	0.958
	1.054
160ng/L	0.474	0.465	0.414
	0.455
80ng/L	0.248	0.212	0.162
	0.176
40ng/L	0.093	0.104	0.054
	0.115
0ng/L	0.051	0.05	0
	0.049

Gene ID	22410
UniProt ID	P48614

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110208	E2397Mo	48T	$320		Add to Cart
BHE12110208	E2397Mo	96T	$458		Add to Cart

Mouse Protein Wnt-10B - WNT10B ELISA Kit

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