Mouse Protein S100-A9(S100A9) ELISA kit

Mfr.No.

CSB-EL020642MO

Sensitivity

0.11 ng/mL

Detection Range

0.45 ng/mL-30 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B

Recovery

The recovery of human SULT1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-95
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SULT1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-89
1:2	Average %	103
	Range %	98-107
1:4	Average %	88
	Range %	84-92
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.078	2.148	2.113	1.918
800	1.512	1.468	1.490	1.295
400	1.004	1.020	1.012	0.817
200	0.733	0.689	0.711	0.516
100	0.496	0.511	0.504	0.309
50	0.381	0.374	0.378	0.183
25	0.275	0.286	0.281	0.086
0	0.197	0.192	0.195	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P31725

Applications	ELISA
Research Area	S100A9

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506548	CSB-EL020642MO	96T	$595	Add to Cart
BHE10506548	CSB-EL020642MO	5x96T	$2856	Add to Cart
BHE10506548	CSB-EL020642MO	10x96T	$5652.50	Add to Cart

Mouse Protein S100-A9(S100A9) ELISA kit

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