Mouse Protein notum homolog(NOTUM) ELISA kit

Mouse Protein notum homolog(NOTUM) ELISA kit

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Mfr.No.

CSB-EL015955MO

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

notum pectinacetylesterase homolog (Drosophila), notum pectinacetylesterase homolog, notum pectinacetylesterase homolog (Drosophila), notum pectinacetylesterase homolog

Recovery

The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-94
EDTA plasma (n=4)	94	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-91
1:2	Average %	92
	Range %	83-99
1:4	Average %	101
	Range %	93-107
1:8	Average %	96
	Range %	90-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.407	2.457	2.432	2.268
500	1.768	1.736	1.752	1.588
250	1.065	1.098	1.082	0.918
125	0.704	0.735	0.720	0.556
62.5	0.448	0.469	0.459	0.295
31.2	0.323	0.300	0.312	0.148
15.6	0.221	0.229	0.225	0.061
0	0.161	0.167	0.164	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8R116

Applications	ELISA
Research Area	NOTUM

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506538	CSB-EL015955MO	96T	$695	Add to Cart
BHE10506538	CSB-EL015955MO	5x96T	$3336	Add to Cart
BHE10506538	CSB-EL015955MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.