Mouse Protein Fam3D - FAM3D ELISA Kit

Mfr.No.

E2388Mo

Sensitivity

0.153ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

FAM83D;Protein FAM83D

Molecular Weight

64324

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2388Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	32.7	1.27	3.9
Intra-Assay	2	18	18.2	1.07	5.9
Intra-Assay	3	18	10.8	0.59	5.5

Background

Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC. May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade. May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.

Protocol

These standard curves of E2388Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.239	2.22	2.196
	2.201
24ng/mL	1.418	1.43	1.406
	1.442
12ng/mL	0.785	0.776	0.752
	0.768
6ng/mL	0.421	0.408	0.385
	0.396
3ng/mL	0.176	0.185	0.161
	0.193
0ng/mL	0.026	0.024	0
	0.021

Gene ID	71878
UniProt ID	Q9D7I8

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110199	E2388Mo	48T	$320		Add to Cart
BHE12110199	E2388Mo	96T	$458		Add to Cart

Mouse Protein Fam3D - FAM3D ELISA Kit

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