Mouse Protein disulfide-isomerase(P4HB) ELISA kit

Mouse Protein disulfide-isomerase(P4HB) ELISA kit

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Mfr.No.

CSB-EL017342MO

Alternative Names

prolyl 4-hydroxylase, beta polypeptide, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, collagen prolyl 4-hydroxylase beta, glutathione-insulin transhydrogenase, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4

Reactivity

Mouse

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse IL22RA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-97
EDTA plasma (n=4)	95	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL22RA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-97
1:2	Average %	100
	Range %	95-103
1:4	Average %	95
	Range %	89-100
1:8	Average %	89
	Range %	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.412	2.423	2.418	2.258
1500	1.812	1.776	1.794	1.634
750	1.116	1.078	1.097	0.937
375	0.705	0.688	0.697	0.537
187.5	0.434	0.459	0.447	0.287
93.75	0.322	0.314	0.318	0.158
46.88	0.256	0.243	0.250	0.090
0	0.162	0.158	0.160	?

Species

Mus musculus

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P09103
Antigen	prolyl 4-hydroxylase, beta polypeptide

Applications

ELISA

Research Area

P4HB

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506525	CSB-EL017342MO	96T	$695	Add to Cart
BHE10506525	CSB-EL017342MO	5x96T	$3336	Add to Cart
BHE10506525	CSB-EL017342MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.