Mouse Protein delta homolog 1(DLK1) ELISA kit

Mouse Protein delta homolog 1(DLK1) ELISA kit

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Mfr.No.

CSB-EL006945MO

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

delta-like 1 homolog (Drosophila), DLK, FA1, PREF1, Pref-1, ZOG, pG2, delta-like 1 homolog, delta-like homolog, fetal antigen 1, preadipocyte factor 1, secredeltin

Recovery

The recovery of pig NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	94	83-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-102
1:2	Average %	90
	Range %	85-99
1:4	Average %	95
	Range %	87-98
1:8	Average %	94
	Range %	86-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.754	2.682	2.718	2.614
25	2.017	1.997	2.007	1.903
12.5	1.330	1.362	1.346	1.242
6.25	0.944	0.957	0.951	0.847
3.12	0.653	0.641	0.647	0.543
1.56	0.378	0.369	0.374	0.270
0.78	0.240	0.251	0.246	0.142
0	0.102	0.105	0.104

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q09163

Applications	ELISA
Research Area	DLK1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	IMP2/p62 induces genomic instability and an aggressive hepatocellular carcinoma phenotype Kessler SM. et al,Cell Death Dis,2015 Oct 1;6:e1894.

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SKU	Supplier No.	Size	Your price
BHE10506521	CSB-EL006945MO	96T	$695	Add to Cart
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BHE10506521	CSB-EL006945MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.